Inhibition:Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear.
Article Snippet: .. Tenocyte stimulation and inhibition assays For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY-P79222, 1 μg/mL); TGF-β1 +OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi +OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pretreatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear
Article Snippet: .. For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY- P79222 , 1 μg/mL); TGF-β1 + OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi + OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pre-treatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Control:Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear.
Article Snippet: .. Tenocyte stimulation and inhibition assays For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY-P79222, 1 μg/mL); TGF-β1 +OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi +OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pretreatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear
Article Snippet: .. For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY- P79222 , 1 μg/mL); TGF-β1 + OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi + OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pre-treatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Article Title: Yiqi Qingre Gao alleviates renal fibrosis in UUO mice via PI3K/AKT pathway
Article Snippet: Cells were treated with transforming growth factor-β1 (TGF-β1) (PeproTech, 100-21) at a concentration of 10 ng/mL and 10% YQQRG medicated serum, which was identified as the optimal concentration. .. The experimental groups included: (1) control group, (2) TGF-β1 group, (3) YQQRG group, (4) Forsythiaside group (MCE, HY-N0028, 30 μM), (5) Cynaroside group (MCE, HY-N0540, 30 μM), and (6) Ononin group (MCE, HY-N0270, 30 μM), with all treatments lasting 24 h. Additionally, the PI3K/AKT agonist 740Y-P (MCE, HY-P0175A) was used at a concentration of 20 μM. ..
Recombinant:Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear.
Article Snippet: .. Tenocyte stimulation and inhibition assays For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY-P79222, 1 μg/mL); TGF-β1 +OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi +OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pretreatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear
Article Snippet: .. For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY- P79222 , 1 μg/mL); TGF-β1 + OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi + OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pre-treatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Incubation:Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear.
Article Snippet: .. Tenocyte stimulation and inhibition assays For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY-P79222, 1 μg/mL); TGF-β1 +OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi +OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pretreatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear
Article Snippet: .. For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY- P79222 , 1 μg/mL); TGF-β1 + OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi + OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pre-treatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Expressing:Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear.
Article Snippet: .. Tenocyte stimulation and inhibition assays For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY-P79222, 1 μg/mL); TGF-β1 +OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi +OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pretreatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear
Article Snippet: .. For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY- P79222 , 1 μg/mL); TGF-β1 + OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi + OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pre-treatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Western Blot:Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear.
Article Snippet: .. Tenocyte stimulation and inhibition assays For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY-P79222, 1 μg/mL); TGF-β1 +OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi +OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pretreatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Article Title: Single cell atlas decodes the molecular dynamics of scar repair after human rotator cuff tear
Article Snippet: .. For TGF-β1 and OPN stimulation assay, primary rat tenocytes were assigned to four groups: Control; TGF-β1 group, treated with recombinant TGF-β1 protein (MedChemExpress, HY-P7117, 10 ng/mL); OPN group, treated with recombinant OPN protein (MedChemExpress, HY- P79222 , 1 μg/mL); TGF-β1 + OPN group, treated with both recombinant TGF-β1 (10 ng/mL) and OPN (1 μg/mL) for 48 h. For TGFβRi and OPNn inhibition assay, cells were assigned to four groups: Control; TGF-β receptor inhibitor (TGFβRi), pre-treated with SB-431542 (MedChemExpress, HY-10431,10 μmol/L) for 1 h; OPN neutralizing antibody (OPNn), pre-treated with OPNn (Bioxcell, BE0382, 10 μg/mL) for 1 h; TGFβRi + OPNn, pre-treated with both SB-431542 (10 μmol/L) and OPNn (20 μg/mL) for 1 h. Following pre-treatment, all groups were stimulated with TGF-β1 (10 ng/mL) and OPN (1 μg/mL) simultaneously while maintaining the inhibitors in the medium, and incubated for 48 h. ECM-related genes expression was then measured by western blotting. ..
Concentration Assay:Article Title: Yiqi Qingre Gao alleviates renal fibrosis in UUO mice via PI3K/AKT pathway
Article Snippet: Cells were treated with transforming growth factor-β1 (TGF-β1) (PeproTech, 100-21) at a concentration of 10 ng/mL and 10% YQQRG medicated serum, which was identified as the optimal concentration. .. The experimental groups included: (1) control group, (2) TGF-β1 group, (3) YQQRG group, (4) Forsythiaside group (MCE, HY-N0028, 30 μM), (5) Cynaroside group (MCE, HY-N0540, 30 μM), and (6) Ononin group (MCE, HY-N0270, 30 μM), with all treatments lasting 24 h. Additionally, the PI3K/AKT agonist 740Y-P (MCE, HY-P0175A) was used at a concentration of 20 μM. ..
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